Effect f Enamel Matrix Derivative n Alkaline Phosphatase Activity n Osteoblast Cells Through he Mitogen-activated Protein Kinase Pathway

The study examined the response of MG63 to Enamel Matrix Derivative (EMD) on the coarse-grit-blasted and acid-etched surface disks. We characterized associated changes in proliferation and differentiation related intracelluar signaling molecular including mitogen activitied protein kibase. The major groups of MAPKs (ERK1/2, cJUN-terminal kinases, JNK and p38-MAPK) have been characterized in previous experiments. We will check which pathways stimulate the proliferation and differentiation of MG63 on the coarse-grit-blasted and acid-etched surface disks by the western blot. EMD regulated the proliferation and differentiation of MG63 on the coarse-grit-blasted and acid-etched surface disks. EMD inhibit the cell proliferation of MG63, increase the differentiation of MG63 and production of osteocalcin and TGF-β1. The signaling pathway induced by END from ERK1/2 JNK and p38 were detected. EMD has been successfully used to affect the proliferation and differentiation of osteoblast on the surface of implants. EMD can accelerate the regeneration of soft and hard tissue in the peri-implantitis disease. EMD can be used in the pero-implantitis disease to regenerate the soft and